Effects of UE on normal fibroblast cells intoxicated (for 24 hours) in vitro

Cells were stained by H+E for this study.

Normal human fibroblasts that received UE from the first day of culture (A), show a great preservation of their morphology and a significant resistance to the colchicine intoxication applied in this in vitro test in moderate toxic doses. The untreated cells (B) were clearly more affected and show a decrease in number and morphological changes in the remaining cells.

Effects of UE on normal human fibroblasts intoxicated in vitro

Cells were stained by H+E for this study.

(A,B) show human fibroblasts treated with moderate dose of colchicine (1X) for 24 hours. Colchicine induced moderate toxicity in cells that did not receive any UE treatment (B). When cells received UE transfers from their first day in culture (A), they preserved their morphology much better than the untreated cells in (B).

(C,D) show cell intoxication with a stronger dose of colchicine (5X). Untreated cells were highly affected by this dose (D), while UE-treated cells (C) show more resistance to the intoxication and less loss in number.

Effects of UE on normal human fibroblasts intoxicated in vitro

Cells were stained by H+E for this study.

(A,B) show human fibroblasts treated with 5X colchicine for 4 hours.

(A) shows the toxic effect on cells not treated with UE (arrows). (B) shows that UE-treated cells are much less intoxicated than untreated cells, and demonstrate minor changes (arrows).

Moreover they appear very similar to the control cells (C) which did not receive any toxic substance.

Normal human fibroblasts intoxicated with colchicine or with partially inactivated colchicine by a 30-second UE treatment by C6

Cells were stained by H+E for this study.

(A) shows in this highly magnified view that fibroblasts remained healthy looking or very slightly affected after the application of colchicine which was directly treated with UE by C6 before being applied.

(B) shows the classic toxic effects of native (untreated) colchicine on fibroblasts. Many cells were affected. The doses used in these experiments were of 5X and no cells received any UE transfer.

These results demonstrate the inactivating effects of UE on toxic substances, when the substance is directly treated by C6 before being applied. Although this inactivation is partial, 40% (see Figure 17B), it is substancial enough to ensure the survival of a number of cells.